|
| 產(chǎn)地 | 中國(guó) |
| 品牌 | Solarbio |
| 貨號(hào) | M1020 |
| 用途 | MTT可以被線粒體內(nèi)的一些脫氫酶還原生成結(jié)晶狀的深紫色產(chǎn)物formazan，在特定溶劑存在的情況下，可以被完全溶解 |
| 包裝規(guī)格 | 500T |
| CAS編號(hào) | |
| 純度 | 現(xiàn)詢客戶% |
| 是否進(jìn)口 | 否 |
MTT細(xì)胞增殖及細(xì)胞毒性檢測(cè)試劑盒
貨號(hào)：M1020
產(chǎn)品名稱 包裝
MTT溶液 5ml
Formazan溶解液 60ml
產(chǎn)品簡(jiǎn)介：
MTT可以被線粒體內(nèi)的一些脫氫酶還原生成結(jié)晶狀的深紫色產(chǎn)物formazan。在特定溶劑存在的情況下，可以被完全溶解。然后通過(guò)酶標(biāo)儀可以測(cè)定490nm波長(zhǎng)附近的吸光度。細(xì)胞增殖越多越快，則吸光度越高；細(xì)胞毒性越大，則吸光度越低。
保存條件：
MTT溶液-20℃保存；Formazan溶解液室溫或-20℃保存。
注意事項(xiàng)：
1.由于使用96孔板進(jìn)行檢測(cè)，如果細(xì)胞培養(yǎng)時(shí)間較長(zhǎng)，一定要注意蒸發(fā)的問(wèn)題。一方面，由于96孔板周?chē)蝗θ菀渍舭l(fā)，可以采取棄用周?chē)蝗Φ霓k法，改加PBS，水或培養(yǎng)液；另一方面，可以把96孔板置于靠近培養(yǎng)箱內(nèi)水源的地方，以緩解蒸發(fā)。
2.MTT溶液為黃色需避光保存，長(zhǎng)時(shí)間光照會(huì)導(dǎo)致失效。當(dāng)顏色變?yōu)榛揖G色時(shí)，請(qǐng)勿使用。Formazan溶解液結(jié)凍或產(chǎn)生沉淀時(shí)可以37℃水浴孵育以促進(jìn)溶解，并且必須在全部溶解并混勻后使用。
3.為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
使用說(shuō)明：
1.收集對(duì)數(shù)期細(xì)胞，調(diào)整細(xì)胞懸液濃度，分于96孔板，每孔180μl，3000-10000個(gè)/孔。
2.置37℃、5%CO2溫箱培養(yǎng)使細(xì)胞貼壁，培養(yǎng)6-24小時(shí)。
3.加入適當(dāng)濃度的受試化合物，繼續(xù)培養(yǎng)適當(dāng)時(shí)間。
4.小心吸去上清，加入90μl新鮮培養(yǎng)液，再加入10ul MTT溶液，繼續(xù)培養(yǎng)4 h。
5.然后吸掉上清，每孔加入110ul二甲基亞砜，置搖床上低速振蕩10 min，使結(jié)晶物充分溶解。在酶聯(lián)
免疫檢測(cè)儀490 nm處測(cè)量各孔的吸光值。
6.同時(shí)設(shè)置調(diào)零孔（培養(yǎng)基、MTT、二甲基亞砜），對(duì)照孔（細(xì)胞、相同濃度的藥物溶解介質(zhì)、培養(yǎng)液、
MTT、二甲基亞砜），每組設(shè)定3復(fù)孔。
北京索萊寶科技有限公司，供應(yīng)細(xì)胞培養(yǎng)相關(guān)產(chǎn)品以及其他生化試劑，實(shí)驗(yàn)、科研用品，參看如下產(chǎn)品類(lèi)目，歡迎咨詢！
產(chǎn)品類(lèi)目：
1.細(xì)胞培養(yǎng)試劑,2.細(xì)胞培養(yǎng)耗材,3.蛋白質(zhì)提取,4.蛋白質(zhì)純化,5.蛋白質(zhì)定量,6.蛋白質(zhì)電泳,7.蛋白質(zhì)Marker,8.Western blot,9.免疫組化,10.抗體,11.SABC試劑盒,12.基因組和質(zhì)粒提取試劑盒,13.核酸提取相關(guān)試劑,14.DNA電泳,15.DNA Marker,16.RNA相關(guān)試劑,17.PCR,18.Southern blot,19.基因工程,20.生物培養(yǎng),21.堿基與核酸,22.氨基酸,23.多肽及蛋白質(zhì),24.酶,25.輔酶,26.酶抑制劑,27.維生素,28.抗生素,29.染色劑,30.糖與碳水化合物,31.生物緩沖液,32.植物生長(zhǎng)激素,33.酸和鹽分離效,34.胺,35.層析介質(zhì),36.常用生化試劑,37.其他生化試劑,38.耗材,39.標(biāo)準(zhǔn)品,40.新增產(chǎn)品
參考文獻(xiàn)：
《Collagen facilitates the colorectal cancer stemness and metastasis through an integrin/PI3K/AKT/Snail signaling pathway》 作者:Xiangbin Wu，Jianhui Cai，Zhigui Zuo，Jinlei Li 期刊:Biomedicine & Pharmacotherapy 影響因子:3.743 PMID:30913493
《Gallic acid has anticancer activity and enhances the anticancer effects of cisplatin in non?small cell lung cancer A549 cells via the JAK/STAT3 signaling pathway》 作者:Tingxiu Zhang, Lijie Ma, Pengfei Wu, Wei Li, Ting Li, Rui Gu, Xiaoping Dan, Zhiwei Li, Xianming Fan, Zhenliang Xiao 期刊:Oncology Reports 影響因子:3.041 PMID:30747218
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression》 作者:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xueping Han, Jie Yu, Wenjie Wang, Lirong Liang, Zheng Liu, Yi Zheng, Jianming Hu, Lan Yang, Jun Li, Hong Zhou, Xiaobin Cui and Feng Li 期刊:Molecular Cancer 影響因子:10.679 PMID:30609930
《Low-intensity pulsed ultrasound in combination with SonoVue induces cytotoxicity of human renal glomerular endothelial cells via repression of the ERK1/2 signaling pathway》 作者:Xiu Liu, Bei Wang, Hongyu Ding, Hao Shi, Ju Liu & Hongjun Sun 期刊:Renal Failure 影響因子:1.687 PMID:30122107
《Apoptosis of human hepatocellular carcinoma cells SMMC-7721 induced by C-3 methylidene thiazolidinedione acetic acid》 作者:Hong-Xia Liang, Yi-Hua Yu, Xiao-Hua Li, Nai-Fu Tang, Guo-Qiang Hu, Bin Liu 期刊:Int J Clin Exp Med 影響因子:0.181 PMID:
《Tumor-associated macrophages promote bladder tumor growth through PI3K/AKT signal induced by collagen》 作者:Qiu Shi, Deng Linghui, Xinyang Liao, Ling Nie 期刊:Cancer Science 影響因子:4.751 PMID:31120174
《Blockade of integrin β3 signals to reverse the stem-like phenotype and drug resistance in melanoma》 作者:Xiaoxia ZhuXiaohua TaoWei LuYang DingYi Tang 期刊:Cancer Chemotherapy and Pharmacology 影響因子:2.808 PMID:30627776
《LncRNA SNHG15 acts as a ceRNA to regulate YAP1-Hippo signaling pathway by sponging miR-200a-3p in papillary thyroid carcinoma》 作者:DM Wu, S Wang, X Wen, XR Han, YJ Wang 期刊:Cell death & Disease 影響因子:5.638 PMID:30237435
《Effect of cell culture models on the evaluation of anticancer activity and mechanism analysis of the potential bioactive compound, iturin A, produced by Bacillus subtilis》 作者:H Zhao, X Zhao, S Lei, Y Zhang, D Shao, C Jiang 期刊:Food & function 影響因子:3.289 PMID:30778489
《Carbon dots serve as an effective probe for the quantitative determination and for intracellular imaging of mercury(II)》 作者:Fanyong Yan,Depeng Kong,Yunmei Luo,Qianghua Ye,Juanjuan He,Xingfei Guo,Li Chen 期刊:Microchimica Acta 影響因子:5.705 PMID:
《Effects of tumor-specific antigen induced by lentinan on murine H22 hepatocellular carcinoma immunoprophylaxis》 作者:Y. WANG1, X. HAN， Y.-D. LI， S.-Y. ZHAO, D.-J. ZHANG,Z.-H. ZHAO, Y.-B. WANG 期刊:European Review for Medical and Pharmacological Sciences 影響因子:1.093 PMID:26698247
《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu 期刊:Surgical Oncology 影響因子:2.136 PMID:
《Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes》 作者:Shicheng Bi, Xiaodan Ma, Yuemin Wang, Xiaoqing Chi, Yong Zhang, Wei Xu, and Songhua Hu 期刊:Oxidative Medicine and Cellular Longevity 影響因子:4.868 PMID:31178974
《Diterpene pekinenal from euphorbia pekinensis radix induced IEC-6 cells apoptosis mediated by mitochondria and death receptors》 作者:Fang Yao,Dongjing Jiang,Fangfang Cheng,Weifeng Yao,Peidong Chen,Shuchen Guo,Yudan Cao,Li Zhang 期刊:Toxicology in Vitro 影響因子:3.067 PMID:30710624
《The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis》 作者:Ke Xue , Jun Zhang , Cong Li , Jing Li , Cong Wang, Qingqing Zhang, Xianlu Chen , Xiaotang Yu , Lei Sun ,Xiao Yu 期刊:Journal of Cellular and Molecular Medicine 影響因子:4.658 PMID:30983140
《TRPC5?induced autophagy promotes the TMZ?resistance of glioma cells via the CAMMKβ/AMPKα/mTOR pathway》 作者:Yan Zou, Mu Chen, Shuai Zhang ,Zeng'li Miao, Jing Wang ,Xiao'jie Lu ,Xu'dong Zhao 期刊:Oncology Reports 影響因子:3.041 PMID:30942446
《Curcumin analogues attenuate Aβ25-35-induced oxidative stress in PC12 cells via Keap1/Nrf2/HO-1 signaling pathways》 作者:Jialin Xu，Leilei Zhou，QiWeng，Linxia Xiao，Qingyong Li 期刊:Chemico-Biological Interactions 影響因子:3.407 PMID:30946834
《3D carbon nanosphere and gold nanoparticle-based voltammetric cytosensor for cell line A549 and for early diagnosis of non-small cell lung cancer cells》 作者:Huan Zhang，Hong Ke，Yinfang Wang，Pengwei Li，Chusen Huang，Nengqin Jia 期刊:Microchimica Acta 影響因子:5.479 PMID:30569315
《Autocrine secretions enhance radioresistance in an exosome?independent manner in NSCLC cells》 作者:Shuang Wang， Piaoyang Gao ，Na Li ，Ping Chen ，Jinhan Wang， Ningning He，Kaihua Ji， Liqing Du ，Qiang Liu 期刊:International Journal of Oncology 影響因子:3.571 PMID:30387839
《Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa》 作者:Hai-yu Ji,Pei Chen,Juan Yu,Ying-ying Feng,An-jun Liu 期刊:Applied Biochemistry and Biotechnology 影響因子:2.14 PMID:30536035
《Cucurbit[7]uril enhances photosensitization of porphyrins in Neuroblastoma cells》 作者:Xiaoyu Li,Bo Xiao,Yan Guo,Yan Xiao,Song Xiao 期刊:Photodiagnosis and Photodynamic Therapy 影響因子:2.589 PMID:30648639
《Scutellarin protects human retinal pigment epithelial cells against hydrogen peroxide (H2O2)-induced oxidative damage》 作者:Xin Hu, Xiaofang Wu, Bo Zhao and Yongyi Wang 期刊:Cell & Bioscience 影響因子:3.355 PMID:30680088
《1-Hydroxy-3,7,8-Trimethoxyxanthone Suppresses the Malignant Proliferation of Human Bone Marrow Mesenchymal Stem Cells in Colon Cancer Microenvironment》 作者:Hui-qiao Zhao, Nian-hua Lu, Xu-dong Zhang, Na Liu and Ming Jing 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF‐κB pathway》 作者:Jianwei Liu , Tongmeng Jiang, Mingwei He , Depeng Fang, Chong Shen, Yiguan Le , Maolin He , Jinmin Zhao, Li Zheng 期刊:Journal of cellular physiology 影響因子:4.522 PMID:30370694
《Honokiol inhibits breast cancer cell metastasis by blocking EMT through modulation of Snail/Slug protein translation》 作者:Wen-die Wang, Yue Shang, Yi Li & Shu-zhen Chen 期刊:Acta Pharmacologica Sinica 影響因子:4.01 PMID:31235819
《Effects of nanoparticle size on the interaction between zinc oxide nanoparticles and bovine serum albumin》 作者:Liwei Wang, Tingting Liang, Jun Ma, Liqian Sun, Congcong Yang, Liqiang Meng et al. 期刊:Journal of Biomolecular Structure and Dynamics 影響因子:3.31 PMID:30938584
《NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis》 作者:Shu-Cai Bai,Qian Xu, Hui Li, Ya-Fei Qin, Li-Cheng Song, Chen-Guang Wang, Wen-Hao Cui, Zhi Zheng,De-Wen Yan,Zhi-Jun Li, Dong Li, Xin Wan,and Hua-Feng Zhang 期刊:Oxidative Medicine and Cellular Longevity 影響因子:4.868 PMID:31049140
《Activation of the p38/MAPK pathway regulates autophagy in response to the CYPOR-dependent oxidative stress induced by zearalenone in porcine intestinal epithelial cells》 作者:Tongtong Shen,Yufan Miao,Chenchen Ding,Wentao Fan,Shuhui Liu,Yanan Lv,Xiaona Gao,MartheDe Boevre,Liping Yan,Sheila Okoth,Sarah De Saeger,Suquan Song 期刊:Food and Chemical Toxicology 影響因子:3.775 PMID:31173817
《miR-9-5p modulates the progression of Parkinson’s disease by targeting SIRT1》 作者:Zuobo Wang，Lin Sun，Kaixue Jia，Hongxia Wang，Xiuxiang Wang 期刊:Neuroscience Letters 影響因子:2.173 PMID:30826419
